Yan Jun, Tang Dahai, Xuan Weidong, Fang Zhengping, Hu Shuigen
School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Department of Pharmaceutics, Liberation Army 411 Hospital
Abstract: Objective: To observe the effects of thesium chinense extracts for treating rat models of IgAN, in order to identify effective parts. Method: Thesium chinense was extracted by decoction and absorbed by AB-8 macroporous resin; different parts were eluted and prepared by ethanol at gradients. Rats orally took BSA (400mg/kg) and were administered castor oil via subcutaneous injection. Rats were modelled by CC14 in combination with lipopolysaccharide, which was administered through tail intravenous injection. From the 8th week, rats of the positive group [(LGT, 10mg/kg), water extract group (TT, 1.0g/kg), group of water soluble parts (TTW, 0.4g/kg), group of polar parts (TT20, 0.4g/kg) and group of moderately polar parts (TT50, 0.4g/kg) were administered via stomach-lavaging. Normal saline was administered to the control group and the model group (n=8) for 5 consecutive weeks. 24-hour urinary volume, 24-h urinary protein, red blood cell count of urinary sediments were observed for different groups of rats. At the end of the experiments, tests of biochemical indicators of blood, immunofluorescence tests and pathological examinations of renal tissues were performed. The results were compared with the control group. It was found that 24-h urinary protein, red blood cell count in urinary sediments, and the content of creatinine and urea nitrogen were significantly higher in the model group on average. Compared with the model group, the 24-h urinary protein (P<0.05)m red blood cell count in urinary sediments (P<0.01), Cr and UN level (P<0.05)declined in the TT and TT50 groups. Less immune complexes were deposited in mesangial regions of renal tissues in different administered groups, where glomerulus was enlarged, mesangial matrix proliferated and injuries of uriniferous tubules were relieved. Conclusion: Water extracts of thesium chinense and their moderately polar parts were effective for treating rats with IgA. They are more effective than tripterygium glycosides of the control group.
Keywords: Thesium Chinense; IgA; Renal Diseases; SD Rats
Authors: Yan Jun, Tang Dahai, Xuan Weidong, Fang Zhengping, Hu ShuigenJournal: Journal of Pharmaceutical Practice, 1st Edition, 2015, 28-31, P35.